D2Dx™ Immunity Test
One-Minute Blood Test for Animal Immune Health Testing
Searching for a reliable and accurate method to evaluate your animals' immune health? Our rapid blood test for animal immune health testing offers a quantitative analysis of the function of your animal's immune system, enabling you to make informed decisions regarding their care and well-being. Count on our cutting-edge technology to provide you with the results you need to keep your animals healthy and happy. Order your Blood Test for Animal Immune Health Testing today!
For human and non-human primates research: while our products are primarily used for livestock and companion animal immune health testing, our products can be used for human and non-human primates related research applications. These research applications include but not limited to, studying the pharmacokinetics of immune stimulating or inhibiting drug and drug candidates; supporting new vaccine development and trials; investigating immune function-related human diseases and drug development, etc. Please CONTACT US for more details.
ABOUT US
Nano Discovery Inc. is a biotechnology company based in Orlando, Florida, United States. We develop, manufacture and sell a rapid blood test product, D2Dx™ immunity test, to detect and monitor livestock and companion animals' immune health. We also supply products for research and studies related to human diseases. D2Dx™ technology is protected by multiple granted patents and pending patent applications.
TECHNOLOGY
D2Dx™ immunity test is a rapid blood test for animal immune health testing. It is a one-of-its-kind test that allows quick and accurate assessment of an animal's immune status on the farm, on the animal side. Producers, farmers and veterinarians can conduct the test in local facilities without shipping samples to laboratories and waiting days to receive the results. Our product is built upon and supported by years of research. Read our PUBLICATIONS AND NEWS to learn more today!
APPLICATIONS
CALVES (DAIRY, BEEF, CROSS)
D2Dx™ immunity test can be used to monitor the immune status and development of dairy, beef, and dairy-beef cross calves at any growth stage, from birth to weaning, to feedlot and finishing. The test provides the information you need to make informed treatment and nutrition management decisions.
- Identify newborn dairy calves with failure of passive immune transfer (FPIT), predict the mortality risk of the neonatal calves
- Identify calves with compromised immune health due to stress (transportation, weaning, heat), and calves with active infections.
- Select and adjust feed, feed additives/supplements and nutrition products that will keep animals in best immune health and reach the best production performance
DAIRY COWS
Infectious diseases (mastitis, metritis) are the leading cause to financial loss in dairy farms. Dairy cows are more prone to diseases (mastitis, metritis) during the peri-parturient period because their immunity is compromised during this period. D2Dx™ immunity test provides a convenient tool to evaluate the cows' immune health on the farm. The test results can help farmers and producers select the best feed/nutrition products and treatments to maximize the immune protection in the cows. Cows with better immune health post calving are more productive and less prone to diseases. Some studies also show that cows with higher immunity test scores during breeding season tend to have higher conception rate from cows with lower immunity scores.
POULTRY
The intensive production system in poultry farms makes infectious diseases particularly challenging to deal with. Due to close contacts, birds are constantly exposed to a wide range of high risk and contagious pathogens. There is also an increasing pressure to reduce the use of antibiotics in livestock industry. To overcome these challenges, the most ideal solution is to boost the animals' own immune health. There are many products (feed, feed additives, supplements, and other non-antibiotic products) that are designed and developed to help improve the birds' immune health. D2Dx™ immunity test provides a convenient tool for producers and nutritionists to monitor the flock's immune health and to select the best performing products for the flock.
SHEEP AND GOATS
D2Dx™ immunity test can be used to monitor the immune status and health in ewes, lambs, and goats. Studies have found that ewes with higher D2Dx™ immunity test scores tend to have less risk of parasitic infection (lower FEC) and lower somatic cell count (LogSCC) in milk compared to ewes with lower immunity scores. D2Dx™ immunity test may also be used to identify mother ewes with better immune health for selective breeding. Research has found a positive correlation between mother ewes' immunity scores during the peri-parturient period and the general health of newborn lambs. Lambs born from mother ewes with higher immunity scores tend to have higher immunity scores and higher average daily gains in the first 60 days following birth.
DOGS AND CATS
The immune health of your pets directly impacts the overall health, well-being and happiness of your beloved animal companions. Due to genetic factors, aging, unbalanced or poor nutrition, your pets' immune health may be compromised. This will increase their risk of infections and other diseases. Studies have shown that D2Dx™ immunity test is able to evaluate the humoral immunity in dogs and cats. Based on the results, proper nutritional products and other intervention solutions may be selected and adopted to improve the immune health of your pets. In the case of an infection, the test helps diagnose the infection early, and guide the selection and use of antibiotics and anti-viral medicines to achieve the best treatment effect.
PRODUCTS
The prices listed here are only for the United States, Canada, and Mexico. Please get in touch with us for a price quote for all other countries and regions. Discounts are available for academic researchers, non-profit organizations and small business owners. Please contact us for more information.
CT-100
CT-100 is the device used to perform the D2Dx™ immunity test. One-time purchase item.
$1500.00/unit
D2Dx™ Immunity Test Kits
D2Dx-kt-100
Test kit that contains the reagent and consumables to perform 100 tests. The kits are used for measuring the immune health of cattle, sheep, goats, broiler chicken, certain types of fish, dogs and cats.
$12.00/test
100 tests per kit
D2Dx-kt-200
Test kit that contains the reagent and consumables to perform 200 tests. The kits are used for measuring the immune health of cattle, sheep, goats, broiler chicken, certain types of fish, dogs and cats.
$11.00/test
200 tests per kit
D2Dx-kt-500
Test kit that contains the reagent and consumables to perform 500 tests. The kits are used for measuring the immune health of cattle, sheep, goats, broiler chicken, certain types of fish, dogs and cats.
$10.00/test
500 tests per kit
D2Dx-ENH-100
Test kit to measure the immune health of swine and some other animal species. Please contact us for additional technical support. The kit contains the reagent and consumables to perform 100 tests.
$15.00/test
100 tests per kit
D2Dx-QT-XXXX
For larger quantities of D2Dx™ immunity test kits, please contact us to request quote. We provide test kit packages with less than 100 tests. A $20-30 special packaging fee may apply.
Quote
PUBLICATIONS AND NEWS
Chris Johnson, John T Richeson, Jennifer Rudd, Janeen L Salak-Johnson, David L Lalman, Rosslyn Biggs, Jared Taylor, Paul A Beck. 187 Does selection of beef cattle for production traits impact post-weaning immunological phenotype and robustness traits. J. Animal. Sci. Volume 103, Issue Supplement_2, June 2025, Page 3.
https://doi.org/10.1093/jas/skaf170.003
Cassie L Smithyman, M M M Smithyman, M E E Branine , G C C Duff , Clint A Löest. PS-21 Evaluation of a rapid immunity test for morbidity and mortality in newly received feedlot cattle. J. Animal. Sci. Volume 103, Issue Supplement_2, June 2025, Page 181.
https://doi.org/10.1093/jas/skaf170.203
Ansley M. Roper, C. G. Savegnago, Thiago N. Marins, Jing Gao, Rui Xie, Sha Tao and Qun Huo. Evaluating an immunity test to predict calf mortality. Biology - A Special Issue on Immune Response Regulation in Animals, 2025, 14, 584.
https://www.mdpi.com/2079-7737/14/6/584
Hansan, D.; Ford, H.R.; Bionaz, M. Impact of maternal diet on the antioxidant status, immune function, and whole-blood selenium levels of lamb offspring. Italian J. Anim. Sci. 2025, 24, 738-752.
https://doi.org/10.1080/1828051X.2025.2468344
Ndegwa, E.; Elhadedy, D.E.; Richey, C.; Kim, C.; Yousuf, A.B. Differential age-based response induced by a commercial probiotic supplementation in pastured goats. Probiotics and Antiomicrobial Proteins 2024, s12602-024-10337-w
https://link.springer.com/article/10.1007/s12602-024-10337-w
Menta, P.R.; Prim, J.; de Oliveira, E.; Lima, F.; Calvão, A.N.; Noyes, N.; Ballou, M.A.; Machado, A.S. Predictive models for metritis cure using farm-collected data, metabolic and inflammation biomarkers, and hemogram variables measured at diagnosis. J. Dairy Sci. 2024, 107, 5016-5028.
https://www.sciencedirect.com/science/article/pii/S0022030224005253
Spring, J.; Huo, Q.; Jimenez, E.; Martinez, M.; Hovingh, E.; Lawhead, J.; Barragan, A.A. Assessment of the effect of prepartum anti-inflammatory therapies on type1/type 2 immunity ratio using a rapid blood test. J. Dairy Sci. Communications, 2024, 5, 144-149.
https://www.jdscommun.org/article/S2666-9102(23)00123-0/fulltext
Ford, H.R.; Hasan, D.; Ates, S.; Puerto-Hernandez, G.M.; Klopfenstein, J.; Trevisi, E.; Smallman, M.; Matra, M.; Bionaz, M. Feeding Chicory Silage, But Not Se-Yeast or a Single Injection of Inorganic Se, Affects Metabolism, Fat in Milk, and Type I Immunity in Transition Ewes. Front. Anim. Sci. 2024, 5:1499480
https://www.frontiersin.org/journals/animal-science/articles/10.3389/fanim.2024.1499480/full#hsm
Smercheck, D.T.; Branine, M.E.; McGill, J.L.; Hansen, S.L. Effects of supplemental Zn concentration and trace mineral source on immune function and associated biomarkers of immune status in weaned beef calves received into a feedlot. J. Animal. Sci. 2023, 101, 1-12.
https://academic.oup.com/jas/article/doi/10.1093/jas/skac428/6966915
Casper D.P. PSX-B-9 A nanotechnology based immune measurement (D2Dx) compared with the total serum protein concentration using neonatal Holstein bull calves. J. Anim. Sci. 2022, 100 (Suppl 3), 218-218.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493626/
Messman, R.D. Evaluating the role of the bovine vaginal microbiome in neonatal and maternal health outcomes. Doctoral dissertation (chapter 3), 2023, Mississippi State University.
https://www.proquest.com/docview/2852116187/fulltextPDF/9075D19783F44D73PQ/1?accountid=10003
Corpron, M.; Ahmadzadeh, A.; Rezamand, P.; Huo, Q. Relationship between beef cow fertility and maternal immunity. Appl. Anim. Sci. 2021, 37, 733-737.
https://www.sciencedirect.com/science/article/pii/S2590286521001452
Lowe, B.; Zheng, T.; Reinhardt, C.; Branine, M.E. PSII-1 An Evaluation of a nutrient drench administered to calves at arrival into the feedlot on subsequent health status and assessment of immune status with a rapid immunity test. J. Anim. Sci. 2022, 100 (Suppl 4), 24-25.
https://academic.oup.com/jas/article/100/Supplement_4/24/6769367
Ford, H.R.; Zheng, T.; Bionaz, M.; Huo, Q.; Hasan, D. The D2Dx immunity test as a measure of immune health in ewes and lambs. J. Dairy Sci. 2022, 105 (Suppl 1), 163.
https://www.journalofdairyscience.org/issue/S0022-0302(22)X7004-5
Messman, R.D.; Swanson, R.M.; Lemley, C.O. 71 Effects of periparturient vaginal betadine lavages on dam and neonatal immune responses. J. Anim. Sci. 2023, 101 (Suppl 1), 58-59.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10158174/
Carter, K.; Weaver, E.; Levesque, C.L. 28 On-Farm rapid test to assess immunological health of young pigs. J. Anim. Sci. 2023, 101 (Suppl 2), 255-256.
https://academic.oup.com/jas/article/101/Supplement_2/255/7332506
Ndegwa, E.; Richey, C.; Ali, D. PSV-21 - Evaluation of the effect of daily probiotic administration on the prevalence of Shiga toxigenic E.coli and select serum and immune parameters during pre and peri-weaning period in goat kids. J. Anim. Sci. 2023, 101 (Suppl 3), 359.
https://academic.oup.com/jas/article/101/Supplement_3/359/7372682
Montgomery, T. Effect of interferon Tau and pregnancy specific protein B on gene expression during early pregnancy. MS degree thesis, Penn State University library. July 22, 2022.
https://etda.libraries.psu.edu/catalog/21528txm5650
Smithyman, M.M.; Branine, M.E.; Sowers, C.A.; Duff, G.C.; Loest, C.A. PSVI-16 - An evaluation using a visual scoring system with vaginal temperatures of newly received feedlot heifers on immune status. J. Anim. Sci. 2024, 102 (Suppl 3), 544-545.
https://doi.org/10.1093/jas/skae234.611
Kimbrough, M.M. Effects of bovine maternal appeasing substance and/or metaphylaxis with tulathromycin in high risk, newly received feedlot heifers. West Texas A&M University, Thesis and Dissertations, August 2024.
https://wtamu-ir.tdl.org/items/5ff09ff4-23cd-4a6b-84fc-7421dd1a9d4c
Kimbrough, M.M.; Schneid, K.N.; Young, J.D.; Samuelson, K.L.; Richeson, J.T. Effects of bovine maternal appeasing substance and/or metaphylaxis with tulathromycin in high risk, newly received feedlot heifers. Plains Nutrition Council 2024 spring conference, April 10-12, 2024.
https://theplainsnutritioncouncil.com/past-proceedings/ (2024, page 133)
Leach, M.A.; Tucker, H.L.M.; Tucker, H.A.; Seymour, W.; Eckelkamp, E.; Sheperd, E.; Mathew, D.; McLean, K.J. 391 - The impact of trace mineral source on systemic immunological status associated with early embryonic loss in lactating dairy cattle. J. Anim. Sci. 2024, 102 (Suppl 3), 288-289.
https://doi.org/10.1093/jas/skae234.330
Hamilton, H.E.; Shepherd, E.; Zoca, S.M.; Dahlen, C.R.; Snider, A.P.; McLean, K.J. PSI-13 - The impact of divergent nutritional planes on systemic immune status in mature bulls. J. Anim. Sci. 2024, 102 (Suppl 3), 470-471.
https://doi.org/10.1093/jas/skae234.533
Mccracken, F.B; Dickerson, S.M.; Skaggs, P.M.; Timlin, C.L.; Fowler, J.W.; Coon, C.N. PSI-15 - Validation of a rapid test to evaluate humoral immunity in labrador retrievers across multiple life stages. J. Anim. Sci. 2024, 102 (Suppl 3), 478.
https://doi.org/10.1093/jas/skae234.543
Savegnago, C.G.; Roper, A.M.; Marins, T.N.; Gao, J.; Cunha, G.G.; Kant, N.L.P.; Czaykowsky, C.R.; Credille, B.; Huo, Q.; Tao, S. Abstract 2698 - Impact of heat stress abatement on humoral immunity in preweaned calves prior to and following bovine respiratory disease. J. Dairy Sci. 2024, 107 (Suppl. 1), 425.
Tsai, C-Y.; Hassan, R.; Hung, H-C.; Weber, T.; Price, W.J.; Rezamand, P.; Huo, Q. A rapid blood test to monitor the immune status change in dairy cows and to evaluate their disease risk during periparturient period. Sensors International, 2021, 2, article 100078.
https://www.sciencedirect.com/science/article/pii/S2666351120300784
Deb, C.; Salinas, A.N.; Zheng, T.; Middleton, A.; Kern, K.; Penoyer, D.; Borsadia, R.; Hunley, C.; Abomoelak, B.; Mehta, V.; Irastorza, L.; Mehta, D. I.; Huo, Q. A 1-minute blood test detects decreased immune function and increased clinical risk in COVID-19 patients. Sci. Rep. 2021, 11, article 23491.
https://www.nature.com/articles/s41598-021-02863-2
Zheng, T.; Crews, J.C.; McGill, J.L.; Khunal, D.; Finn, C.; Strutt, T.M.; McKinstry, K.K.; Huo, Q. A single-step gold nanoparticle-blood serum interaction assay reveals humoral immunity development and immune status of animals from neonates to adults. ACS Infectious Diseases, 2019, 5, 228-238. https://pubs.acs.org/doi/10.1021/acsinfecdis.8b00213
CONTACT US
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